HPLC chromatography column/Hypercarb liquid chromatography column/Thermo Fisher spare parts/Thermo Hypercarb HPLC chromatographic column

100% porous graphite carbon for expanding separation function

• Excellent retention ability for highly polar analytes.

Separate substances with similar structures.

• Maintain stability at pH 0 to 14.

Suitable for high-temperature applications.

HPLC chromatography column/Hypercarb liquid chromatography column/Thermo Fisher spare parts/Thermo Hypercarb HPLC chromatographic column

Porous graphite carbon (PGC) is a unique stationary phase formed by arranging carbon atoms in a sheet-like hexagonal shape. The valence of these carbon atoms is already saturated, similar to most multi-core aromatic molecules. The structure and retention properties of Hypercarb are different from traditional silica gel bonding phases, with a wide range of pH stability and the ability to retain and separate highly polar compounds.

HPLC chromatography column/Hypercarb liquid chromatography column/Thermo Fisher spare parts/Thermo Hypercarb HPLC chromatographic column

Interaction mechanismIt mainly depends on the polarity and planarity (shape) of the solute. These specific interaction mechanisms enable the successful retention and separation of analytes that cannot be separated by conventional reverse phase HPLC. Due to the absence of complex buffer systems or ion pair reagents when analyzing polar analytes, as well as the use of higher concentrations of organic modifiers, compatibility with detection techniques such as MS is also higher.

Hypercarb chromatography columns are retained through two mechanisms:

1. Adsorption: The strength of the interaction between the analyte and Hypercarb largely depends on the molecular area in contact with the graphite surface, as well as the type of functional group at the contact point and the position of the functional group relative to the graphite surface. The right figure shows the way in which planar and non planar molecules approach the surface of Hypercarb. The strength of the interaction depends on the size and direction of the molecular area that can come into contact with the surface of flat graphite. Molecules with higher planarity have higher retention than rigid molecules arranged in three-dimensional space.

2. The interaction between charge induced polar analytes and polarizable graphite surfaces: When polar groups with * dipoles approach the surface, they will form induced dipoles, thereby enhancing the mutual attraction between the analyte and the graphite surface. These charges should not be confused with the total ionic charge of molecules, such as alkaline compounds ionized under acidic pH conditions. The charge induced dipole mechanism is entirely caused by the interaction between the electrostatic charges of polar molecules and the surface of graphite.